Diabetes Predict uses the gold standard technology of TaqMan® OpenArray® for genetic analysis. It is an automated process, with a maximum processing capacity in a minimum analysis time and is low-cost, which guarantees optimum quality of the results obtained in the genotype of the DNA. This technology allows the amplification of certain fragments of DNA where the polymorphisms to be studied are situated. The use of high specificity probes linked to fluorophores allows the detection of the genetic variants present in each individual analysed.
The test uses TaqMan probes methodology which is based on the nuclease activity 5′-3′ of the Taq polymerase enzyme and the detection of the genetic variants with a fluorophore. Each test contains two fragments of nucleotides (primers) which specifically join the regions flanking the SNP, and two TaqMan fluorescent probes with a connection to the minor groove (MGB) of the DNA helix. Each probe is marked with a different fluorophore (VIC or 6-FAM) covalently tied to the 5′ end of each of the two probes. Near the 3′ end, there is a group which prevents the liberation of the fluorescence if the probe is intact. The MGB is used to stabilise the double thread structure formed between the DNA sequence to be examined and the probe.
During the PCR, the probes that specifically hybridise the DNA fragments are destroyed and the fluorescence of the corresponding fluorophore is freed and detected.